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Unlike other antidiabetic drugs that cause the pancreas to secrete more insulin, metformin makes the bodys tissues more sensitive to insulin, thus reducing insulin resistance (Nasri 2014)

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The following antibodies were used: anti-GPX4 (1:2000, Abcam, ab125066), anti-ACSL4 (1:1000, Abcam, ab155282), anti-TfR (1:1000, Invitrogen, H68.4), anti-FPN1 (1:1000, Eurogentec, Lege, Belgium) anti-ferritin (1:1000, Sigma, F5012), anti-phospho-NF-B p65 (1:1000, Cell Signalling Technology, 3039), anti-NF-B p65 (1:1000, Cell Signalling Technology, 8242), with anti-GAPDH (1:2000, Cell Signalling Technology, 2118) or anti--actin (1:2000, Sigma, A2066 and Abcam, ab49900) as loading control

C., Tesfay, L., Torti, S