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Vector and insert fragments were linearized by polymerase chain reaction (PCR) and template DNA was digested with DpnI

Chronic non-communicable diseases (NCDs), including cardiovascular disease, Type 2 diabetes, obesity, metabolic syndrome, and metabolic-associated fatty liver disease, continue to rise worldwide 1

Glutamine triggers and potentiates glucagon-like peptide-1 secretion by raising cytosolic Ca 2+ and cAMP

While tirzepatide slows gastric emptying and reduces caloric intake through incretin receptor activation, 5-amino-1MQ works intracellularly to inhibit nicotinamide N-methyltransferase (NNMT), shifting cellular metabolism toward fat oxidation independent of appetite suppression