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Table 1) harboring roGFP-based sensor protein-coding plasmids (pPT_ roGFP2 or pPT_ roGFP-iL , Supplementary table S2) were cultivated in MOPS minimal medium (Technova, Hollister, CA, USA) at 37 C until an optical density (OD) of 0.50.8 was reached

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However, we do not fully understand why these aging hallmarks occur in the first place, and therefore there have been no proven solutions via human randomized clinical trials to improve or correct aging hallmarks in aging humans. The aging hallmarks that improved are mitochondrial dysfunction, altered intercellular communication, nutrient sensing, loss of proteostasis, genomic instability, cellular senescence and stem cell fatigue

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