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For detection of vinculin as loading control, membranes were blocked with Intercept Blocking Buffer (LI-COR Biosciences, #927-70001) for 1 h at room temperature, incubated with the primary antibody anti-vinculin (Sigma-Aldrich, V9131, RRID: AB_477629, 1:5000) overnight at 4 C in blocking buffer, then washed with PBS-T (PBS with 0.1% Tween-20) five times and incubated with IRDye 800CW-conjugated secondary antibody (LI-COR Biosciences, #926-32210, RRID: AB_621842, 1:5000) in blocking buffer for 1 h at room temperature with protection from light

Among plant-based proteins, soy protein shows GLP-1-enhancing effects and there are many ways to incorporate soy protein into meals

Vitamin C contributes to production of collagen for normal functions of the skin

Figure 5 To further interrogate the anti-inflammatory action of melatonin treatment on ILC2s we cultured ILC2s ex vivo for 24h with either melatonin or vehicle control