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10.1210/en.2015-1551 345
Optimization of anesthesia protocol for resting-state fMRI in mice based on differential effects of anesthetics on functional connectivity patterns
Attaching of N -Boc-norleucine, removal of Boc, acetylation of norleucine amino group with acetic anhydride, HF mediated cleavage of the resulting peptide from the polymer support, and finally purification using RP-HPLC afforded the target compound 2 in 5560% overall yield [18,24]
Further detail is provided by Aroda et al
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