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An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)

The volume added by the lyophilized powder itself is incredibly small and generally considered negligible for dosing calculations

Physiology of growth hormone secretion

296 In addition, in the further study with tumor-bearing mice models, macrophages from mice of different ages exist diverse phenotypes, indicating that aging is accompanied with changes in inflammatory cells and will affect tumor progression