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ScGrx7 is particularly suited for unbiased kinetic and mechanistic studies because of its high activity, the absence of ironsulfur clusters in enzyme preparations and the lack of additional cysteine residues 11 (glutaredoxins with two or more cysteine residues have complex kinetics because of alternative redox states and reactions 10,12,14,17,30,31,32,33 )
For researchers prioritizing tolerability over maximum effect, the 1mg tier provides a meaningful metabolic intervention with minimal disruption
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There are a number of reasons why real-world use and efficacy may differ from observed impacts in RCTs such as the STEP and SUSTAIN trials
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