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To obtain a GLP-1RG s complex with good homogeneity and stability, we used the NanoBiT tethering strategy, in which the C terminus of GLP-1R was directly attached to LgBiT subunit followed by a TEV protease cleavage site and a double MBP tag

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The microdose approach offers a gentler, more affordable entry with minimized side effects, while Nooms behavioral framework increases the odds of lasting results