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2.3.4 Determination of the content of B-group vitamins The content of B vitamins, such as thiamine hydrochloride (vitamin B1), riboflavin (vitamin B2), nicotinic acid (vitamin B3), nicotinamide (vitamin PP), pantothenic acid (vitamin B5), pyridoxine hydrochloride (vitamin B6) and folic acid (vitamin B9) was determined by high performance liquid chromatography coupled with tandem mass spectrometry and electrospray ionization (HPLC-ESI-MS/MS), according to the procedure previously described by Smole et al
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TABLE 3 Jadad scale for randomised controlled trials
This effect is particularly important for individuals with obesity, as sustained weight loss is a key factor in mitigating the risks associated with excessive weight, such as cardiovascular disease, type 2 diabetes, and metabolic syndrome
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