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P., Stenvers, D
Post incubation, the liberated IGF-1 was co-immunoaffinity extracted with the internal reference, using protein A/G MSIA pipette tips (from Thermo Fisher Scientific) loaded with antihuman IGF-1 antibody
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Overall, despite limited research on the effect of short-term overfeeding on macrovascular blood flow in humans, studies looking at large artery dilation suggest that a short-term HFD may impair endothelial function
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