The H-ESI source was operated in positive mode at spray voltage 3,500 or negative mode at spray voltage 2,500 with the following parameters: sheath gas 35 a.u., aux gas 7 a.u., sweep gas 0 a.u., ion transfer tube temperature 320 C, vaporizer temperature 275 C, mass range 70 to 1,000 m/z , full scan MS1 mass resolution of 120,000 FWHM, RF lens at 70% and standard AGC
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Previous studies in rats and small clinical trials involving children with ASD suggest that the drug bumetanide, which has been approved for use in oedema, a condition that results in a build-up of fluid in the body, could help reduce symptoms of ASD
Lipidomic profiling of CE and TG species was performed by mass spectrometry-based analysis
