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If anything, it raises the bar
An 1290 HPLC system (Agilent Technologies, Palo Alto, USA) and a 6460 triple-quadrupole mass spectrometer (Agilent Technologies, Palo Alto, USA) were used
Retatrutide is not FDA-approved and is available only for research purposes
The selection between direct HPLC analysis and indirect GC-FID quantification depends on the specific research requirements, with HPLC preserving the intact triglyceride information while GC-FID often provides superior sensitivity for fatty acid quantification
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