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The separated proteins were loaded and incubated on the PVDF membrane with primary antibodies for Beclin 1 (# PA5-20172), p-ERK (#44-680G), p-mTOR (#44-1125G), p-PI 3 K (# PA5-99367), ERK ( #136200), mTOR (# AHO1232), LC 3 A (#PA5-23180), LC 3 B (#PA1-16930), NFB (p65) (# 510500), and -actin (# MA5-15739) (Thermo Fisher Scientific Inc., Waltham
10,11 Regarding IOP-lowering mechanisms, GLP-1RAs have been shown to inhibit sodium-potassium ATPase in the choroid plexus, decreasing cerebrospinal fluid secretion and lowering intracranial pressure
Tirzepatide acts on both GLP-1 and GIP receptors, providing a broader metabolic effect compared to GLP-1 alone
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