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The excess fluid from the hopper overflows back into the pit
Reconstitution: Gently inject the desired amount of bacteriostatic water into the vial of AHK-Cu
For the B 12 bolus experiment, mice were administered 5 g vitamin B 12 (Sigma, V2876) dissolved in water by oral gavage on day 6 after the start of doxycycline treatment, and blood samples were taken by submandibular collection just before and 24 h after the bolus
Identical to a pre-mixed compounded vial at the same concentration
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