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The V3-V4 region of bacterial 16S rRNA gene was amplified using primers 338 F (5-ACTCCTACGGGAGGCAGCA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3)
The logic is identical: a sub-therapeutic priming dose lets the gut and central receptors acclimatise before the standard ladder begins
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DTPA, diethylenetriaminepentaacetic acid
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