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Effect of increasing the ionic strength and diluting the cell lysate in the presence and absence of BugBuster without sonication Induced cells were next used in triplicates and re-suspended independently in LSB-2 containing 75, 150 or 300 mM NaCl keeping all the other components (10 mM Tris, 1 mM EDTA, 10 mM -mercaptoethanol and 1 mM PMSF) intact, followed by incubation with BugBuster and processed without any sonication

OS specifically reduces HOXA 10 transcriptional activity in stromal cells, likely through mechanisms involving histone modifications or direct disruption of regulatory signaling pathways [11, 51]

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