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Description

Effect of increasing the ionic strength and diluting the cell lysate in the presence and absence of BugBuster without sonication Induced cells were next used in triplicates and re-suspended independently in LSB-2 containing 75, 150 or 300 mM NaCl keeping all the other components (10 mM Tris, 1 mM EDTA, 10 mM -mercaptoethanol and 1 mM PMSF) intact, followed by incubation with BugBuster and processed without any sonication

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OS specifically reduces HOXA 10 transcriptional activity in stromal cells, likely through mechanisms involving histone modifications or direct disruption of regulatory signaling pathways [11, 51]

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