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In addition to the challenging genetic manipulation, the inherent physiological characteristics of CB also serve as crucial limiting factors for the development of ECB
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It directly stimulates pro-opiomelanocortin/cocaine-and-amphetamine-regulated transcript (POMC/CART) neurons and indirectly inhibits neuropeptide-Y/agouti-related protein (NPY/AgRP) neurons through GABA neurotransmission, reducing appetite and promoting weight loss [40]
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