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The elution was concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column that was pre-equilibrated with 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 1 M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS
Practical considerations: AHK-Cu is available in some commercial skincare products, often marketed alongside or as an alternative to GHK-Cu
Frequently asked questions Is GHK-Cu approved as a medicine?+ How do I handle a 100 mg GHK-Cu vial?+ Can the calculator tell me how to use GHK-Cu topically?+ Why are GHK-Cu vials often larger than other peptides?+ Primary sources Full reference list Background references for this calculator
There is no need to aspirate (pull back on the plunger) for subcutaneous injections
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