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Materials and methods HepG2 culture We obtained the human hepatoma HepG2 cell line (HB-8065, ATCC) from ATCC (Manassa, Virginia, USA) and maintained it in Dulbecco's modified Eagle's medium (DMEM) (31966047, Gibco, Massachusetts, USA) at 37 C and 5% CO2

Implementation of this idea led to the creation of what was to be the first contingent of the Manuel Ascunce Domenech Teaching Detachment.1 Through its work in various courses in the schools, this reservoir of future leaders helped to educate thousands of young people at secondary level

GHK-Cu: Antioxidant Protection and Inflammatory Modulation GHK-Cu (glycyl-L-histidyl-L-lysine bound to copper) provides critical antioxidant and anti-inflammatory functions that protect healing tissues from oxidative damage and excessive inflammatory stressfactors that commonly impair regeneration and promote fibrosis

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