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B., Michalek, A
The following antibodies were used for the supernatant A: anti-Akt antibody, anti-Phospho-Akt(Ser473) antibody, anti-Phospho-Akt (Thr308) antibody, anti-PI3 Kinase p85 antibody(Cell Signaling Technology, Danvers, MA), anti-SRD5A1 antibody, and anti-GLUT4 antibody(Abcam, Cambridge, UK).The next day, membranes were incubated with goat anti-rabbit-HRP secondary antibody(Abcam, Cambridge, UK) at room temperature for 1 h
Nunnari, J., & Suomalainen, A
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