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5-Amino-1MQ is a substrate-mimetic inhibitor that occupies the nicotinamide binding site, with a reported IC of 1.2 0.1 M (Neelakantan et al., J Med Chem, 2017)
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The enzyme was dissolved in phosphate-buffered saline at pH 7.4, sterilized through a 0.22-m membrane, and administered under ultrasound guidance
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