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While TRP itself did not show a notable change, its indole-containing metabolite I3AA was significantly elevated, as was the ratio of I3AA/TRP, possibly indicating a shift away from the serotonergic pathway of TRP metabolism
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Additionally, C-incubation time (min) demonstrates a moderate effect with a p-value of 0.0284
Recommended technique: steady pressure over 30 seconds for 0.5 mL, 45 seconds for 1 mL
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