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INS-1 cells were cultured in RPMI-1640 medium containing 11.1 mM glucose supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, 100 g/ml streptomycin, 10 mM HEPES, 2 mM L-Glutamine, 1 mM sodium pyruvate, and 50 M -mercaptoethanol
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Acknowledgements The authors thank Yale Liver Center Morphology Core/Molecular Core Facilities for the support of confocal microscopy, image processing, and biological assays
Marroquin, J
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