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The reaction mixture, which initially contained 570 L of 0.1 M potassium phosphate buffer with 1 mM of EDTA (pH 7.0), 100 L of crude enzyme, one hundred microlitters of 10 mM GSH and 30 L of glutathione reductase from Baker's yeast (50 units of lots [129H7465 and 78H7430]) was incubated at 37C for 10 min