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Briefly, animals were assigned to two groups ( n = 5 male mice per group), vehicle (5% DMSO and 0.5% Kollidon-30 in 100 mM sodium phosphate buffer) or TLC-2716 (1 mg per kg (body weight) daily by oral gavage) for 8 days, after which animals were humanely killed by cardiac puncture, and plasma and tissues were collected for lipid and gene expression endpoints as described below

Images and videos of the droplets and fluid boundaries during rotation were captured using a wireless microscope (SV606, SVBONY, Kowloon, Hong Kong)

5 , 189 (2016) Acknowledgements This work was supported by the National Natural Science Foundation of China grants 31330019 (Z.-J.L.), 31500593 (G.S.), 81373463 and 81573479 (D.Y.), the National Health and Family Planning Commission grants 2012ZX09304-011, 2013ZX09401003-005, 2013ZX09507001 and 2013ZX09507-002 (M.-W.W.), Shanghai Science and Technology Development Fund 15DZ2291600 (M.-W.W.), and Ministry of Science and Technology of China grants 2014CB910400 (Z.-J.L.) and 2015CB910104 (Z.-J.L.), the Netherlands eScience Center (NLeSC)/NWO Enabling Technologies project: 3D-e-Chem, grant 027.014.201 (C.d.G.), the European Cooperation in Science and Technology Action CM1207 GLISTEN (C.d.G.), and National Key Research and Development Program of China 2016YCF0905902 (S.Z.)

The dose most commonly associated with modest weight and fat outcomes is about 1.5 to 2 grams of L-carnitine per day