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A Pierce BCA Assay kit (Thermo Scientific, 23227) was used to measure protein concentration, after which 75 g protein from each lysate was moved to a clean Eppendorf tube and brought to 147 l with the same homogenization buffer
A study of the full program found substantial weight loss, and meta-analyses of lifestyle interventions that include multiple contacts for at least a year find greater losses at one and three years
We have also been studying SLC25A39, but the results are less conclusive and are the subject of ongoing studies
I say this gently
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