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Culture media from 24-hour incubated cells were collected, stored at -80 C, and analyzed for urea concentration using LC/MS/MS API3000, normalized to total cell protein

Its like your bodys saying, Hey, Im not a fan of this! Allergic reactions to glutathione injections can happen due to hypersensitivity to the compound or other additives in the injection solution

Nat Rev Mol Cell Biol 20(1):2137 Gholinezhad M, Sartipi B, Amjadi I (2024) Evaluation of the effects of melatonin-treated RAW macrophage cells in the A-375 melanoma cell line