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The samples were incubated for 30 min at 37 C, and 100 l of 12 N sulfuric acid (H2SO4) was added to stop the enzymatic reaction

In particular, they have shown a reduction of major adverse cardiovascular events (MACE) in diabetic patients at high cardiovascular risk [23,24,25,26,27,28,29,30]

The N-terminal tryptophan makes DSIP modestly light-sensitive in solution, since indole side chains can photo-oxidise another reason to minimise light exposure once reconstituted

However, they are considered to be more effective than oral supplements as they facilitate the direct delivery of compounds into the bloodstream, and by extension, into the skin and other target organs by skipping the digestive process