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After washed twice with PBS in the plates, cells were removed from the plate by scraping twice with each 0.3 ml BHM solution consisting of 0.32 M sucrose, 1 mM EDTA, 10 mM Tris-Cl (pH 7.0) and the complete protease inhibitor cocktail (Roche Applied Science, Upper Bavaria, Germany)
The result tempers claims that DSIP functions as a cortisol-lowering 'anti-stress' peptide in humans and underscores how limited and inconsistent the controlled human endocrine data for DSIP remain
10.1016/j.bbrc.2021.11.029 125 LiH.YuY.LiuY.LuoZ.LawB
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