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2.4 Sample preparation For LCQTOF-MS/MS analysis, 1 mg of powder or homogenized tablet was dissolved or extracted using 5 mL of a 1:1:1 (v/v/v) mixture of water/methanol/acetonitrile, assisted by ultrasonication for 10 min, and afterwards filtered by a Whatman 0.2 m pore size polytetrafluoroethylene (PTFE) filter medium (GE Healthcare, Chicago, IL, United States)

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The pentadecapeptide form sold for research was first characterized by Professor Predrag Sikiri's group at the University of Zagreb in the early 1990s, and the synthetic material supplied here is produced by solid-phase peptide synthesis to match that defined sequence