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For quantification of sulfur-containing metabolites in cultured cancer cells and mouse serum, 10 l samples were extracted with 90 l of ice-cold extraction solvent (80% methanol and 20% H 2 O) containing isotope-labelled internal standards (36 M 13 C 2 15 N-GSH, 0.92 M 13 C 4 15 N 2 -GSSG and 10 M D 4 -cystine)
BMC.Neurol
The key factor is consistency in avoiding overstimulation while engaging in appropriate effortful activities
storage is governed by the IMP label, the sponsor's Pharmacy Manual, and GCP guidelines, with the trial site pharmacist as the definitive authority
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