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B12 is an essential water-soluble vitamin that the body cannot produce, requiring dietary intake or supplementation It acts as a cofactor for enzymes involved in DNA synthesis, red blood cell production, and neurological function Deficiency causes megaloblastic anaemia and can lead to irreversible neurological damage including demyelination B12 is naturally found almost exclusively in animal-based foods

It is interesting to note that an increase was only observed in with a decrease of pH from 7.4 to 5.0 and a negligible change was observed in at studied pH (Kobayashi et al

Lyophilized preparations should remain sealed and stored at -20C away from light and moisture