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The gradual dose escalation used in trials is clinically important it is designed to reduce the incidence and severity of nausea, vomiting, and other gastrointestinal adverse effects that are common with this class of medication
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After quantification of RNA with NanoDrop 2000 (Thermo Fisher), cDNA was synthesised by mixing RNA with FS buffer, DTT (Thermo Fisher), and Random Primers (Sigma-Aldrich) and incubated for 3 min at 70 C
Oral is 50 100 mg daily
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