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A prepacked Ni-NTA column was equilibrated with dialysis buffer and the flowthrough was collected and concentrated to 2 mL using a 10 kDa MWCO concentrator (Amicon Ultra 15, Millipore)
Avoid repeated freezethaw cycles as they degrade peptides over time [11]
Tissue scarring harbors low-grade inflammation that disrupts normal endometrial receptivity and decreases implantation probability
Less Effective in Severe Cases When neurological symptoms are present, rapid correction may be necessary
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