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For the final steps of mutant construction and confirmation, recombinant colonies were selected by plating on 7H10-ADC with the appropriate antibiotic/supplement and confirmed by PCR using specific primers (Supplementary Table 2 and Fig

Each peptide binds to a receptor on or inside a cell, initiating cascades like growth, repair, fat metabolism, inflammation regulation, or hormone signaling

Chlorambucil is an effective alkylating agent used in cancer treatment, targeting DNA replication and inducing apoptosis

Studies of Glutathione show that a healthy cell should have over 100 times more reduced glutathione than its oxidized form