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Animals were anaesthetized with ethyl ether after which a single intracolonic dose of 30 mg of TNBS dissolved in 0.25 ml of either 50% ethanol in saline (v/v) or saline, referred as TNBS+ethanol or TNBS+saline, respectively, was administered

The challenges encountered with behavioral interventions alone necessitate understanding why early pharmacological intervention may be warranted

For each 5mg vial, add 1mL of sterile water slowly down the side of the vial, then gently swirl until dissolved

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