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Total proteins were extracted with protein extraction buffer (100 mM Tris-HCl pH 8, 150 mM NaCl, 10% glycerol, 5 mM EDTA, 10 mM DTT, 2 mM PMSF, 10 mM NaF, 10 mM Na 2 MoO 4 , 2 mM NaVO 3 , 1%(v/v) NP-40, 1%(v/v) plant protease inhibitor cocktail)
The recommended daily intake of niacin for lactation is ~17-20 mg, which is easily met by a normal diet
The grapefruit brings a tangy sweetness, along with a beautiful pink hue
6 is a liquid chromatogram of Compound 4 obtained in example 5
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