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These concentrations affect both paracellular and transcellular pathways and are associated with membrane fluidisation and intracellular calcium signalling cascades involving phospholipase C, IP 3 , and MLCK, which trigger cytoskeletal contraction and TJ opening (Tomita et al., 1996
Next, the lysis buffer was added to disrupt cell membranes and release intracellular constituents
alternative protocols (e.g., 100 mcg every other day) may be selected based on clinical response and laboratory indices
Third, it modulates inflammatory signalling and supports healthy immune function
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