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By stimulating the growth of blood vessels, AHK-Cu may potentially influence growth
Normal dermal fibroblasts were seeded into a 96-well cell culture plate and have been grown to confluence in high glucose media, supplemented with 10% fetal calf serum for around 3 days
Transforming growth factor-beta 1 (TGF-beta1) induces angiogenesis through vascular endothelial growth factor (VEGF)-mediated apoptosis
Further experimentation involved Annexin V-fluorescein isothiocyanate/propidium iodide labeling and flow cytometric analysis, which suggested that AHK-Cu may have reduced the number of apoptotic DPCs, although this decrease was not statistically significant and thus not conclusively confirmed
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