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Using another batch of purified protein, kindly gifted by Dr Zachary Lee Johnson and Dr Jue Chen (Laboratory of Membrane Biology and Biophysics, The Rockefeller University, New York, USA), we once again showed that S-verapamil binds MRP1 with a similar affinity of 130 30 nM, in agreement with our previous results 11
Its structure was confirmed by mass spectrometry and amino acid analysis [2]
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