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The supernatant obtained after centrifugation was used to determine the protein-bound form of glutathione and related peptides
The study revealed three key engineering challenges in heterologous hydrolase expression systems, including (1) growth impairment caused by metabolic burden, (2) host protease-dependent enzyme degradation, and (3) suboptimal transmembrane transport of polymeric substrate degradation intermediates
doi:10.1093/toxres/tfac050 Hashmi, M
Pfaus JG, Sadiq A, Spana C, Clayton AH
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