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(PubMed) Terry EN, Diamond JA
We confirmed both in vitro and in vivo that specific combination of ligands of different transporters, especially alanine and glutathione, is more efficient than single ligands to facilitate the penetration of nanocarrierss across the BBB [24, 32, 33, 42]
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Centrifuge the sample at 15,000 g at 4 C for 30 min, then dissolve the dry pellet in extraction buffer, thoroughly mixed by vortex and then placed in cold 0.1 M ammonium acetate - methanol solution for 12 h at 20 C
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