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Crypts were released after vigorous shaking in cold PBS and passed through a 70 M cell strainer (BD Biosciences) for further enrichment
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The results showed that maternal RPM supplementation in late gestation promoted more significant global DNA methylation in the offsprings liver, accompanied by a general alteration in the hepatic transcriptome, proteome, and metabolome, favoring the enrichment in glucose and lipid metabolism, glutathione, and immune system pathways (Palombo et al., 2021)
202 For example, acatalasemia, the state in which there is a virtually total absence of red cell catalase, is devoid of hematologic manifestations
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