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The cycle threshold (CT) values of gene expression were normalized to the housekeeping gene -actin, and the expression ratios were calculated using the CT method

This thermal effect is complemented by oxidative stress pathways involving lipid peroxidation and glutathione depletion, which are exacerbated by resveratrols pro-oxidant activity at high concentrations [75, 76]

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Activation of FXR stabilizes the nuclear co-repressor (NcoR) at the NF-B response element on the IL-1 promoter, while decreasing the expression of TNF-, IL-1, IL-2, IL-6 and IFN- mRNA, thereby reducing the extent of inflammation (Vavassori et al., 2009)