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Consequently, NRF2 dissociates from Keap1, escapes ubiquitination and degradation, accumulates in the cytoplasm, and ultimately translocates to the nucleus [85, 91, 92]

Just how much of a challenge this posed can be gauged from the fact that Mutt's group needed 10km of porcine small intestine to obtain about 1mg of pure secretin (Rehfeld 2012)