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Neutralization of the eluted proteins with neutralizing buffer (1 M Tris, pH 10.4) may help preserve its activity
The Keap1-BTB protein is an adaptor that bridges Nrf2 to a Cul3-Based E3 ligase: oxidative stress sensing by a Cul3-Keap1 ligase
doi:10.3389/fendo.2024.1384603 Raparelli V, Nocella C, Proietti M, et al
Low levels of the zinc-dependent enzyme alkaline phosphatase (ALP)---included in a comprehensive metabolic panel---may help reveal a functional zinc deficiency
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