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and DNA synthesis

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Western blot analysis and Coomassie Brilliant Blue (CBB) staining Samples were boiled in sample loading buffer at 100 C for 10 min, separated by SDS-PAGE, and transferred onto NC membranes for blotting

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Gene analysis also revealed that exendin-4 treatment increased the bone formation markers ALP, osteocalcin, and P1NP, while decreasing the bone resorption parameter CTX (43)

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