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The small volume of acetic acid introduced at this stage is generally inconsequential at final working concentrations, but researchers should always confirm that the final assay pH and buffer conditions are appropriate for the specific experimental model being used

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In addition, Caveolin-1 (Cav-1), a 21-kDa integral plasma membrane protein is a well-known negative regulator of eNOS activity in vascular endothelial cells 17,18,19,20,21,22

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