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Addressing these limitations will necessitate coordinated advancements across scientific research, regulatory frameworks, and clinical methodologies
Stress-responsive eccDNA effects can be highly context dependent, making multi-site, multi-year validation essential to ensure durable efficacy without unintended ecological impacts
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However, the absence of DsbA shifted the periplasmic thiol-redox potential from -228 mV to a more reducing -243 mV and the capacity to re-oxidize periplasmic roGFP2 after a reductive pulse was significantly decreased
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