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ReverTraR Ace qPCR RT Master Mix with gDNA Remover and Blend TaqR was utilized to produce cDNA and to amplify the synthesized cDNA (Toyobo Co., Ltd., Science Department, Osaka, Japan)

Bioflavonoids are compounds found in citrus fruits that help the body use Vitamin C more effectively

Wild-type (ECACC) and -arrestin-less HEK293 cells 41 stably expressing SNAP-GLP-1R were generated by G418 selection and maintained in DMEM at 25 mM d-glucose supplemented with 10% FBS and 1% penicillin/streptomycin

For injectable GLP-1 medications, this typically means dissolving the API in a sterile solution, adjusting the pH and tonicity, adding preservatives or stabilizers as needed, performing sterile filtration, and filling the solution into vials or syringes under aseptic conditions